In vitro Culture of Western Redcedar (Thuja plicata Donn). I. Plantlet Formation

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Abstract
Juvenile and mature tissues of T. plicata were induced to form multiple adventitious buds when cultured in continuous light at 25.degree. C on defined nutrient media containing 1 .times. 10-6 M to 1 .times. 10-5 M BAP [6-benzylaminopurine] in the presence or absence of 1 .times. 10-7 M NAA [naphthaleneacetic acid]. Excision and transfer of adventitious buds onto a wide range of defined and undefined media allowed shoot elongation and leaf formation to occur. Subsequent transfer of the shoots onto media containing 5 .times. 10-6 M to 5 .times. 10-5 M IBA induced adventitious root formation within 4-6 wk. The resulting plantlets exhibited normal shoot morphology and were successfully transferred from tissue culture flasks to greenhouse conditions.

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