Observations on the Growth Phases of Human Amnion Cell Cultures2

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Abstract
Human amnion cells in their first and second subculture underwent 3 phases: 1) a limited initial proliferative, 2) a prolonged stationary and degenerative, and 3) a slow recovery phase. The initial proliferative phase lasted about 6–10 weeks; in most cultures, the stationary and degenerative phase about 20–42 weeks. In the initial phase, cell number increased threefold to tenfold. In the stationary and degenerative phase there was a slow decrease in cell number, and the mitotic activity was extremely low or nondetectable by the methods employed. In the recovery phase, foci of proliferating cells appeared and enlarged slowly but persistently for several months. In 7 experiments, 29 of 32 hydrocortisone-treated cultures showed evidence of recovery between the 22d and 52d week. Addition of hydrocortisone to nutrient medium appeared crucial in bringing the amnion culture into the recovery phase regularly.

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