Incorporation of oxygen-18 into nucleosides and bases
Open Access
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (6) , 2075-2082
- https://doi.org/10.1093/nar/4.6.2075
Abstract
Extensive incorporation of oxygen-1818at position 04of the pyrimidine nucleus results from exchange between H20 and nucleosides or bases in IN HC1 at 100°. The reaction is hindered By substitution at C-5 with the greatest effect shown in pseudouridine (R = ribosyl) and the least in uri-dine (R = H). Maximum incorporation in the latter compound was 98%, and in uricil was 983. The method is experimentally simple and the incorporation is readily monitored by mass spectrometry.Keywords
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