A mucus‐secreting human colonic epithelial cell line responsive to cholinergic stimulation

Publisher Website
Google Scholar
Abstract
The human colonic epithelial cell line C1.16E grows in culture as a polarized monolayer which differentiates at confluency into typical goblet cells secreting their mucin content into the culture medium. Polyclonal antibodies raised against these mucins were used in an ELISA to measure the amount of mucins secreted by the C1.16E cells. Carbachol caused a transient and significant increase in mucus secretion with a maximal stimulation occuring at 30 min. A dose-dependent effect was found with a maximal stimulation with 10-3M carbachol. This effect was inhibited by atropine. These results indicate that the effects of carbachol are mediated by msucarinic receptors present on mucus-secreting epithelial cells.