Antigen Excess in Guinea Pig Anaphylaxis

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Abstract
Summary: Systemic anaphylaxis in passively sensitized guinea pigs failed to occur when the animals were challenged with massive doses (0.5 to 1.0 g) of protein antigen intracardially. In the isolated sensitized guinea pig lung, bronchospasm was diminished or absent when large amounts of antigen were used for perfusion. PCA in guinea pig skin passively sensitized with rabbit anti-DNP sera was markedly reduced when large concentrations of divalent DNP haptens (bis-DNP lysine and bis-DNP ornithine) were employed. These observations suggest that the inhibition of anaphylaxis by large concentrations of antigen may depend upon the formation in vivo of “nontoxic” complexes of composition Ag2Ab.