Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine

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Abstract
A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethyHhymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphite-triester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed.