T cell‐mediated cytotoxic immune responsiveness of chimeric mice bearing a thymus graft fully allogeneic to the graft of lymphoid stem cells

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Abstract
Fully allogeneic, chimeric mice were established by adult thymectomy of (A × B) F1animals, grafting parental A‐type thymus under the kidney capsula, followed by lethal (900 rd) irradiation and reconstitution with B parental‐type bone marrow cells treated with xenogeneic anti‐T cell antiserum plus complement. Following in vivo sensitization with inactivated Sendai virus (SV) suspensions, no virus‐specific T cells could be detected within the spleen cells of the mice. Upon stimulation with third‐party allogeneic cells in a primary mixed lymphocyte culture, spleen cells of all animals generated alloreactive cytotoxic T lymphocytes (CTL). More interestingly, upon secondary in vitro stimulation with inactivated SV‐conjugated B‐type stimulator cells, B‐type‐restricted, virus‐specific CTL were inducible in each case. Upon restimulation with SV‐conjugated A‐type stimulator cells, A being the H‐2 type of the grafted thymus, T cells of some but not all mice generated A‐type‐restricted, virus‐specific CTL. The data suggest that in allogeneic, chimeric mice virus‐specific CTL can be induced. Moreover, virus‐specific CTL, restricted to the H‐2 type of the lymphoid stem cell inoculum, are more readily inducible than those restricted to the H‐2 type of the allogeneic thymus.

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