Conformations of Fibroblast and E. coli-Derived Recombinant Human Interferon-βs as Studied by Nuclear Magnetic Resonance and Circular Dichroism
- 1 April 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 99 (5) , 1533-1535
- https://doi.org/10.1093/oxfordjournals.jbchem.a135623
Abstract
The conformations of fibroblast and E. coli-derived recombinant human interferonβs were studied by circular dichroism and nuclear magnetic resonance spectroscopy in the acidic pH region of 4.6 to 1.6. Both interferons have very similar conformations with high α-helix contents (∼70%). These results suggest that glycosylation does not appreciably change the conformation of human interferon-β. Moreover, a slow conformational change is observed below pH 2.0, which induces the disruption of β-sheets.This publication has 0 references indexed in Scilit: