Acid injury of Escherichia coli

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Abstract
Two strains of Escherichia coli sublethally treated in acidic environments showed an increased sensitivity to violet red bile agar. The death of cells, treated in tryptic soy broth which had been acidified with two inorganic and five organic acids, was followed on both nutrient agar and the selective medium violet red bile agar. The death rates determined on violet red bile agar were much faster than those established on nutrient agar. Therefore, it appeared that unreliable results may occur if selective media are used for the detection and enumeration of Enterobacteriaceae in acid foods.

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