The response regulator RssB controls stability of the sigma(S) subunit of RNA polymerase in Escherichia coli.
- 15 March 1996
- journal article
- Vol. 15 (6) , 1333-9
Abstract
The rpoS-encoded sigma(S) subunit of RNA polymerase is a central regulator in a regulatory network that governs the expression of many stationary phase-induced and osmotically regulated genes in Escherichia coli. sigma(S) is itself induced under these conditions due to an increase in rpoS transcription (only in rich media) and rpoS translation as well as a stabilization of sigma(S) protein which in growing cells is subject to rapid turnover. We demonstrate here that a response regulator, RssB, plays a crucial role in the control of the cellular sigma(S) content. rssB null mutants exhibit nearly constitutively high levels of sigma(S) and are impaired in the post-transcriptional growth phase-related and osmotic regulation of sigma(S). Whereas rpoS translational control is not affected, sigma(S) is stable in rssB mutants, indicating that RssB is essential for sigma(S) turnover. RssB contains a unique C-terminal output domain and is the first known response regulator involved in the control of protein turnover.This publication has 17 references indexed in Scilit:
- Regulation of Escherichia coli starvation sigma factor (sigma s) by ClpXP proteaseJournal of Bacteriology, 1996
- Autoregulation of the Escherichia coli heat shock response by the DnaK and DnaJ heat shock proteins.Proceedings of the National Academy of Sciences, 1993
- KatF (sigma S) synthesis in Escherichia coli is subject to posttranscriptional regulationJournal of Bacteriology, 1993
- The putative sigma factor KatF is regulated posttranscriptionally during carbon starvationJournal of Bacteriology, 1993
- COMMUNICATION MODULES IN BACTERIAL SIGNALING PROTEINSAnnual Review of Genetics, 1992
- Regulation of katF and katE in Escherichia coli K-12 by weak acidsJournal of Bacteriology, 1992
- The DnaK chaperone modulates the heat shock response of Escherichia coli by binding to the sigma 32 transcription factor.Proceedings of the National Academy of Sciences, 1992
- Identification of a central regulator of stationary‐phase gene expression in Escherichia coliMolecular Microbiology, 1991
- Regulation of transcription of katE and katF in Escherichia coliJournal of Bacteriology, 1990
- The osmZ (bglY) gene encodes the DNA-binding protein H-NS (H1a), a component of the Escherichia coli K12 nucleoidMolecular Genetics and Genomics, 1990