SEROLOGICAL STUDIES ON A CASE OF LABORATORY DENGUE INFECTION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 25  (4) , 163-170
Abstract
A patient who had previously been immunized with Japanese encephalitis (JE) vaccine showed symptoms of typical dengue fever 6 days after accidental infeciton with a newly isolated dengue virus type 4 strain from a patient with dengue hemorrhagic fever (DHF) in Thailand. His sera were examined by hemagglutination inhibition (HI), complement fixation (CF) and neutralization (N) tests. The JE N antibody titers of his sera were high even on the 1st day of the illness and remained almost constant during the next year. Antibodies that reacted with dengue viruses were detected from a very early stage of the illness by all 3 serological tests. His convalescent phase sera showed high titers against all 4 types of dengue virus. Apparently, the dengue infection caused secondary stimulation of other flavivirus antigens. Sedimentation analysis of antibodies in the patients serum (day 9) was carried out; IgM antibody that reacted only with dengue type 4 virus and homologous infecting virus was separated. Thus, the laboratory infection of the patient was primary infection with dengue virus type 4.