Concentration of enteroviruses from large volumes of turbid estuary water

Publisher Website
Google Scholar
Abstract
A method is described for the efficient concentration of viruses from large volumes of highly turbid estuary water. Virus in acidified sea water in the presence of aluminum chloride is adsorbed to a 10-in. (about 25.4 cm) fibreglass depth cartridge and 2- and 0.65-μm epoxy-fibreglass filters in series. This filter series is capable of efficiently adsorbing enteroviruses from 50 U.S. gallons (about 190 ℓ) of estuary water of varying salinity and turbidity. Adsorbed viruses were eluted from the filters with glycine buffer (pH 11.5) and the eluate reconcentrated by using a precipitate formed by the addition of ferric chloride. Viruses were eluted from this precipitate with fetal calf serum. Using this procedure, four different enteroviruses in 50 gallons (about 190 ℓ) of estuary water were concentrated 9 000- to 12 000-fold with an overall efficiency of 41%.