A Revised Tissue Culture Medium for Shoot Multiplication of Rhododendron
Open Access
- 1 May 1984
- journal article
- Published by American Society for Horticultural Science in Journal of the American Society for Horticultural Science
- Vol. 109 (3) , 343-347
- https://doi.org/10.21273/jashs.109.3.343
Abstract
Revision of the Murashige-Skoog (MS) 1962 inorganic formula was necessary for the successful culture of rhododendron cultivars. A bioassay system was developed to assess the effects of modifying the MS formula. Inorganic revisions included reduction of nitrogen from 60 to 14.7 mm and potassium from 20 to 4.7 mm. Phosphorus was adjusted to 2.75 mm. Iron concentration was doubled to 200 μm and iodine was reduced to 1.8 μm. These changes reduced the salinity of the inorganics from 5.2 to 2.2 mmho cm−1. General growth regulator concentrations for shoot proliferation are 1 mg/liter 3-aceticindole acid (I A A) and 5 mg/liter N6-(2-isopentenyl)-adenine (2iP), but these concentrations vary among cultivars.Keywords
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