Abstract
The minimal nutritive requirements of 6 spp. of Gram-positive phytopathogenic Corynebacterium were detd. under conditions of controlled inoculum size and carefully prepared glassware and media. All spp. required obligately additions to the NH4Cl, glucose, and salts basal medium, as follows: C. fascians, thiamine; C. flaccumfaciens and C. poinsettiae, amino acids, biotin, pantothenate, and thiamine; C. insidiosum, C. michiganense, and C. sepedonicum, amino acids, biotin, nicotinic acid, and thiamine. Moderate growth-stimulating effects were noted with some strains of C. insidiosum and C. sepedonicum upon additions of yeast extract to the casein-vits. medium.