Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis
- 1 June 1995
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 177 (11) , 3308-3311
- https://doi.org/10.1128/jb.177.11.3308-3311.1995
Abstract
We fused obg, encoding an essential GTP-binding protein in Bacillus subtilis, to the LacI-repressible, IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter Pspac. Depletion of Obg, following removal of IPTG, caused a defect in sporulation and in expression of sporulation genes that are activated by Spo0A approximately P. These defects were significantly relieved by a mutation in spo0A (rvtA11) that bypasses the normal phosphorylation pathway, indicating that Obg might normally be required, either directly or indirectly, to stimulate activity of the phosphorelay that activates Spo0A.Keywords
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