Comparison of the Biophysical and Biochemical Properties of Penicillium cyaneo-fulvum Virus and Penicillium chrysogenum Virus

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Abstract
The biophysical and biochemical properties of P. cyaneo-fulvum virus (Pc-fV) and P. chrysogenum virus (PcV) were compared. In sucrose density gradient sedimentation, purified virus preparations gave 1 major component, L, and 3 minor components E1, E2 and H with sedimentation coefficients of 145S, 80S, 102S and 172S, respectively, in each case. E1 and E2 were empty particles. Pc-fV L particles contained only double-stranded RNA, which separated in polyacrylamide gel electrophoresis into 4 components with MW of 2.21 .times. 106, 2.08 .times. 106, 1.98 .times. 106 and 1.93 .times. 106. PcV L particles gave 3 double-stranded RNA components, which were indistinguishable in polyacrylamide gel electrophoresis from the larger 3 RNA components of Pc-fV. In both viruses each RNA component was separately encapsidated. In both viruses H particles gave rise to the same double-stranded RNA components as their L particles, but contained, in addition, small amounts of single-stranded RNA. Pc-fV and PcV consisted of isometric particles of the same size and were serologically related; the amino acid compositions of their capsid polypeptides were very similar. The capsid polypeptides of the 2 viruses were examined by SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis. In each case E2, L and H particles gave 1 major polypeptide .lambda.1, with a MW of 130,000, whereas E1 particles contained 1 major polypeptide .lambda.2 with a MW of 115,000. The MW of L particles, determined from sedimentation and diffusion coefficients, was 9.8 .times. 106 for both Pc-fV and PcV. The capsid of the L particles of each virus was estimated to contain 60 molecules of polypeptides .lambda.1.

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