VITAMIN REQUIREMENTS OF A LOW-TEMPERATURE BASIDIOMYCETE

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Abstract
The vitamin requirements of an unidentified basidiomycete, the cause of severe snow mold of grasses and legumes in Western Canada, have been determined in shake culture. In a medium composed of D-glucose, L-asparagine, and mineral salts, a mixture of several vitamins supported optimum growth, but an absolute requirement was demonstrated only for thiamine. Pyridoxine stimulated growth in the presence of thiamine. The optimum concentration of thiamine was 50 μg per liter and below this level growth was proportional to concentration. Pyridoxine was effective in the range 50–100 μg per liter in the presence of 50 μg per liter of thiamine. The requirement for thiamine could be satisfied by a mixture of equivalent amounts of thiazole and pyrimidine but not by either alone.