Autofluorescence of vesicles, arbuscules, and intercellular hyphae of a vesicular–arbuscular fungus in leek (Allium porrum) roots

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Abstract
Vesicles, arbuscules and hyphae of a species of Glomus in A. porrum mycorrhizae autofluoresced under 4 different ranges of wavelengths. Autofluorescence persisted when transverse sections were cleared in either 10% KOH or 1% NaOH and was intensified by staining the sections with Chelidonium majus L. root extract. The fluorescing component in the walls of the vesicular-arbuscular mycorrhizal structures was tentatively identified by spectrofluorometry as chitin.