Isolation of proteoglycans from human articular cartilage
- 1 January 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 169 (1) , 123-132
- https://doi.org/10.1042/bj1690123
Abstract
Proteoglycans were extracted from normal human articular cartilage of various ages with 4M-guanidinium chloride and were purified and characterized by using preformed linear CsCl density gradients. With advancing age, there was a decrease in high-density proteoglycans of low protein/uronic acid weight ratio and an increase in the proportion of lower-density proteoglycans, richer in keratan sulphate and protein. Proteoglycans of each age were also shown to disaggregate in 4M-guanidinium chloride and at low pH and to reaggregate in the presence of hyaluronic acid and/or low-density fractions. Osteoarthrotic-cartilage extracts had an increased content of higher-density proteoglycans compared with normal cartilage of the same age, and results also suggested that these were not mechanical or enzymic degradation products, but were possibly proteoglycans of an immature nature.This publication has 30 references indexed in Scilit:
- Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronateBiochemical Journal, 1976
- Proteoglycans of mineralizing rib and epiphyseal cartilageBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- Comparison of proteoglycans from bovine articular cartilageBiochimica et Biophysica Acta (BBA) - Protein Structure, 1975
- STUDIES ON A HUMAN THYROID PROTEASE.1964
- On the interaction between polysaccharides and other macromoleculesBiochimica et Biophysica Acta, 1963
- A modified uronic acid carbazole reactionAnalytical Biochemistry, 1962
- A New Universal Buffer SystemNature, 1961
- The effect of hyaluronic acid on the sedimentation rate of other substancesBiochimica et Biophysica Acta, 1961
- Ultracentrifugal Fractionation of Bovine Nasal ChondromucoproteinJournal of Biological Chemistry, 1960
- The determination of glucosamine and galactosamineBiochemical Journal, 1955