Determination of prostaglandin synthetase activity in rectal biopsy material and its significance in colonic disease.
Open Access
- 30 September 1978
- Vol. 19 (10) , 875-877
- https://doi.org/10.1136/gut.19.10.875
Abstract
A method is described for determining prostaglandin synthetase activity in milligram amounts of tissue. The procedure is based on the conversion of 14C-arachidonic acid to prostaglandin E2 and F2alpha-like substances. High levels of prostaglandin synthetase activity occurred in the inflamed mucosa of patients with ulcerative colitis and fell during successful drug therapy, but it is not yet known whether the cause of the inflammation first involves increased PG synthetase activity, or whether inflammation caused increase of PG synthetase.This publication has 14 references indexed in Scilit:
- PROSTAGLANDINS, ULCERATIVE COLITIS, AND SULPHASALAZINEThe Lancet, 1975
- Radioimmunoassay Measurement of Prostaglandins E, A, and F in Human PlasmaJournal of Clinical Investigation, 1973
- A rapid spectrophotometric assay for prostaglandin synthetase: Application to the study of non-steroidal antiinflammatory agentsProstaglandins, 1972
- Differential inhibition of prostaglandin synthetase and soybean lipoxygenaseProstaglandins, 1972
- Prostaglandin production by mouse fibrosarcoma cells in culture: Inhibition by indomethacin and aspirinBiochemical and Biophysical Research Communications, 1972
- HIGH‐DOSE INTRAVENOUS ADMINISTRATION OF PROSTAGLANDIN E2 AND F2α FOR THE TERMINATION OF MIDTRIMESTER PREGNANCIESBJOG: An International Journal of Obstetrics and Gynaecology, 1972
- Extraction of Prostaglandins from Human BloodNature, 1971
- EFFECTS OF ORAL ADMINISTRATION OF PROSTAGLANDINS E2 AND F2α ON THE HUMAN UTERUSBJOG: An International Journal of Obstetrics and Gynaecology, 1971
- Metabolism of prostaglandin E2 in the ratBiochemistry, 1971
- Inactivation of Prostaglandins by the LungsNature, 1970