ISOLATION OF HIGH-FREQUENCY RECOMBINING STRAINS FROM ESCHERICHIA COLI CONTAINING V COLICINOGENIC FACTOR

  • 1 January 1968
    • journal article
    • research article
    • Vol. 96  (1) , 205-+
Abstract
The isolation and char-acterization of high-frequency recombining strains from different E. coli host cells containing either the F factor or the Col V factor are described. The strains (with 1 exception) formed from 3 of the V+ parents showed the same origin and polarity of transfer (xyl-arg-pro- trp-his-mal). The Hfr strains formed from the 1 remaining V+ and the F+ host cells showed a greater variety in their points of origin. In addition, several Hfr strains isolated from V+ parents lost the ability to produce colicin V. The Fv+ segregants of these were isolated, and the Tv factors appeared to retain their preferential site for Hfr formation, but they lacked other properties controlled by the Col V factor. Chromosomal integration of episomes and its relation to the fertility of F+ and V+ strains is discussed. Production of colicin V appeared to be uninfluenced by the state of the Col V factor within the cell.

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