HISTOCHEMICAL DEMONSTRATION OF CATECHOLAMINE UPTAKE BY ADRENERGIC FIBERS
Open Access
- 1 April 1965
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 13 (4) , 282-285
- https://doi.org/10.1177/13.4.282
Abstract
Uptake of catecholamines by adrenergic fibers was tested in isolated rat irises using the histochemical formaldehyde condensation technique. Minimum concentration in medium for detectable uptake was 0.5 µg/ml for the naturally-occurring catecholamines norepinephrine, epinephrine and dopamine. Adenosine mono-, di-, or triphosphate in minimum concentration of 0.01 µg/ml was required for amine uptake. Parallel specific chemical analyses confirmed the uptake of norepinephrine and epinephrine shown histochemically.Keywords
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