An Anti‐B Reagent Prepared from the α‐D‐Galactopyranosyl‐Binding Isolectins from Bandeiraea Simplicifolia Seeds

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Abstract
A method for the large scale preparation of the .alpha.-D-galactosyl binding isolectins from B. simplicifolia seeds using an absorbent prepared by linking D-galactosamine to CH-Sepharose is described. The addition of N-acetyl-D-galactosamine (GalNAc) to these isolectins produced an anti-B reagent (BS I + GalNAc anti-B). Although BS I + GalNAc anti-B readily agglutinated red blood cells from the majority of group B and AB donor and patient blood samples tested, it reacted weakly with group B and AB cord red blood cells and failed to agglutinate 5 of 100 group AB donor blood samples when tested by an automated technique. The reagent did not agglutinate the red blood cells from 7 acquired B red blood cell samples and was strongly reactive with Tn-polyagglutinable red blood cells. This lectin anti-B preparation may be of more value in investigative immunohematology rather than as an alternative to human group A serum as a source of anti-B for blood-typing purposes. The results of tests on Tn-polyagglutinable red blood cells with BS I + GalNAc anti-B and the purified isolectins BS I(A4) and BS I(B4) were presented and discussed in relation to current concepts on the structure of the Tn receptor.