Production of Microsclerotia by Species of Cylindrocladium

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Abstract
C. scoparium [from diseased roots of yellow-poplar and black walnut seedlings], C. crotalariae, C. floridanum, C. ilicicola and C. parvum were studied to determine factors that affect production of microsclerotia. The most favorable temperature range for growth was 24-28.degree. C. The C/N ratio of the substrate was most important with maximum numbers of microsclerotia formed at ratios of 40:1 and 100:1; fewer were formed at lower ratios on agar media and in sand culture. Potassium nitrate was a good N source at favorable ratios, but poorer than casein hydrolysate at unfavorable ratios. L-Tryosine as a sole source of C and N was excellent for formation of microsclerotia. Straw and sawdust also were good substrates for microsclerotia formation. Evidence suggests that the practice of mulching seed beds known to harbor Cylindrocladium with straw, sawdust or other high-C products may cause a significant increase in numbers of microsclerotia.

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