Production of Monoclonal Antibodies againstLegionella pneumophilaSerogroup 1

Abstract
Four monoclonal antibodies to Legionella pneumophila Philadelphia 1 were produced by the fusion of immunized BALB/c lymphocytes to a murine myeloma cell line. Two (Lpl‐1 and Lpl‐3) of the four monoclonal antibodies reacted with 14 L. pneumophila serogroup 1 strains, and the other (Lpl‐2 and Lpl‐4) reacted with only three out of 20 strains tested. These four monoclonal antibodies did not bind to any strains of L. pneumophila serogroup 2–7 and other Legionella species. In addition, it has been shown that these monoclonal antibodies may be useful not only for subserotyping of L. pneumophila but also for retrospective diagnosis using immunopathological methods.

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