Shuttling of HDAC5 in H9C2 cells regulates YY1 function through CaMKIV/PKD and PP2A
- 1 November 2006
- journal article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 291 (5) , C1029-C1037
- https://doi.org/10.1152/ajpcell.00059.2006
Abstract
YY1 is a transcription factor that can activate or repress transcription of a variety of genes and is involved in several developmental processes. YY1 is a repressor of transcription in differentiated H9C2 cells and in neonatal cardiac myocytes but an activator of transcription in undifferentiated H9C2 cells. We now present a detailed analysis of the functional domains of YY1 when it is acting as a repressor or an activator and identify the mechanism whereby its function is regulated in the differentiation of H9C2 cells. We show that histone deacetylase 5 (HDAC5) is localized to the cytoplasm in undifferentiated H9C2 cells and that this localization is dependent on Ca2+/calmodulin-dependent kinase IV (CaMKIV) and/or protein kinase D (PKD). In differentiated cells, HDAC5 is nuclear and interacts with YY1. Finally, we show that HDAC5 localization in differentiated cells is dependent on phosphatase 2A (PP2A). Our results suggest that a signaling mechanism that involves CaMKIV/PKD and PP2A controls YY1 function through regulation of HDAC5 and is important in the maintenance of muscle differentiation.Keywords
This publication has 41 references indexed in Scilit:
- Yin Yang 1 represses α-myosin heavy chain gene expression in pathologic cardiac hypertrophyBiochemical and Biophysical Research Communications, 2004
- Inactivation of the Myocyte Enhancer Factor-2 Repressor Histone Deacetylase-5 by Endogenous Ca2//Calmodulin-dependent Kinase II Promotes Depolarization-mediated Cerebellar Granule Neuron SurvivalJournal of Biological Chemistry, 2003
- Transcription factor YY1 functions as a PcG protein in vivoThe EMBO Journal, 2003
- Functional Significance of Activation of Calcium/Calmodulin–Dependent Protein Kinase II in Angiotensin II–Induced Vascular Hyperplasia and HypertensionHypertension, 2002
- The Cardiac-specific Nuclear δB Isoform of Ca2+/Calmodulin-dependent Protein Kinase II Induces Hypertrophy and Dilated Cardiomyopathy Associated with Increased Protein Phosphatase 2A ActivityJournal of Biological Chemistry, 2002
- Regulation of Transcription Factor YY1 by Acetylation and DeacetylationMolecular and Cellular Biology, 2001
- Identification of a Signal-Responsive Nuclear Export Sequence in Class II Histone DeacetylasesMolecular and Cellular Biology, 2001
- Unlocking the mechanisms of transcription factor YY1: are chromatin modifying enzymes the key?Gene, 1999
- Competition Between Negative Acting YY1 versus Positive Acting Serum Response Factor and Tinman Homologue Nkx-2.5 Regulates Cardiac -Actin Promoter ActivityMolecular Endocrinology, 1997
- A Novel Highly Specific and Potent Inhibitor of Calmodulin-Dependent Protein Kinase IIBiochemical and Biophysical Research Communications, 1995