Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family
- 1 January 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (5) , 1431-1442
- https://doi.org/10.1093/nar/13.5.1431
Abstract
We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5' and 3' non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.Keywords
This publication has 20 references indexed in Scilit:
- Complete nucleotide sequence of the messenger RNA coding for chicken muscle glyceraldehyde-3-phosphate dehydrogenaseBiochemical and Biophysical Research Communications, 1984
- Plasmid screening at high colony densityGene, 1980
- Prolonged incubation in calcium chloride improves the competence of Escherichia coli cellsGene, 1979
- Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cellsCell, 1978
- Expression of GAPDH and TPI in dog-rodent hybridsCytogenetic and Genome Research, 1978
- Chromosomal assignment and regional localization of CS, ENO2,GAPDH, LDHB, PEPB, and TPI, in man-rodent cell hybridsCytogenetic and Genome Research, 1978
- Gene dosage effect for human triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in partial trisomy 12p13 and trisomy 18pHuman Genetics, 1978
- Biochemical EvolutionAnnual Review of Biochemistry, 1977
- Enzyme polymorphism in feral, outbred and inbred rats (Rattus norvegicus)Heredity, 1976
- Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNAAnalytical Biochemistry, 1976