Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation
Open Access
- 12 July 2004
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (29) , 10572-10577
- https://doi.org/10.1073/pnas.0404195101
Abstract
Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.Keywords
This publication has 45 references indexed in Scilit:
- Crossover/Noncrossover Differentiation, Synaptonemal Complex Formation, and Regulatory Surveillance at the Leptotene/Zygotene Transition of MeiosisCell, 2004
- Imposition of Crossover Interference through the Nonrandom Distribution of Synapsis Initiation ComplexesCell, 2004
- Supercomplex formation between Mlh1–Mlh3 and Sgs1–Top3 heterocomplexes in meiotic yeast cellsBiochemical and Biophysical Research Communications, 2002
- The Mnd1 Protein Forms a Complex with Hop2 To Promote Homologous Chromosome Pairing and Meiotic Double-Strand Break RepairMolecular and Cellular Biology, 2002
- The Single-End InvasionCell, 2001
- Meiosis-Specific DNA Double-Strand Breaks Are Catalyzed by Spo11, a Member of a Widely Conserved Protein FamilyCell, 1997
- Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 ProteinScience, 1994
- Chromosome pairing via multiple interstitial interactions before and during meiosis in yeastCell, 1994
- Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosisCell, 1994
- In vivo processing of N‐terminal methionine in E. coliFEBS Letters, 1990