Expression of H-2 and Moloney Leukemia Virus-Determined Cell-Surface Antigens in Synchronized Cultures of a Mouse Cell Line
- 1 March 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (3) , 566-569
- https://doi.org/10.1073/pnas.68.3.566
Abstract
A mouse cell line derived from bone marrow (JLS-V9) was infected in vitro with Moloney leukemia virus. After the cell-surface antigens specified by this virus appeared, cells were synchronized in mitosis by a short treatment with colcemid. The expression of H-2- and virus-determined surface antigens was monitored during one cell cycle by an indirect membrane-immunofluorescence test. The highest proportion of antigen-positive cells was found during the G1 period; the proportion dropped as the cells entered the S period, and remained low until they entered the G1 period of the next cycle. The H-2- and virus-determined surface antigens were temporally coexpressed.Keywords
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