EOSINOPHILS AND IMMUNE-MECHANISMS .3. PRODUCTION OF LYMPHOKINE EOSINOPHIL STIMULATION PROMOTER BY MOUSE T LYMPHOCYTES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 116  (4) , 1078-1083
Abstract
The lymphoid cell population responsible for production of eosinophil stimulation promoter (ESP), a lymphokine which increases migration of eosinophils, was investigated in murine Schistosoma mansoni infection. Con A [concanavalin A] challenge induced ESP production; Salmonella tyhimurium lipopolysaccharide did not. Prior treatment with anti-.theta.C3H alloantiserum plus [guinea pig] complement in vitro eliminated ESP production; in vivo treatment with rabbit anti-mouse thymocyte serum [ATS] consistently reduced ESP production by splenic lymphoid cells, but affected lymph node cell ESP production only after exceptionally large doses. Thymocytes did not produce significant amounts of ESP; nor did lymphoid cells from congenitally athymic mice. Depletion of bone marrow-derived lymphocytes and macrophages by nylon fiber adherence eliminated antigen-induced ESP production; this was partially restored by addition of non-immune, 72 h peritoneal exudate cells. Con A-induced ESP production was not affected by nylon fiber treatment. ESP is probably produced by an ATS-sensitive, peripheralized thymus-derived lymphocyte population. A macrophage requirement for antigen-induced production of this lymphokine is suggested.

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