A Simplified Procedure for the Preparation of Immunoglobulin-Class-Specific Antisera

Open Access
Publisher Website
Google Scholar
Abstract
A method is described for preparing immunoglobulin-class-specific antisera which requires neither the isolation of homogeneous immunoglobulin preparations for immunization, nor the subsequent absorption of antisera to remove cross-reacting antibody. The method is based on the principle that guinea pigs rendered tolerant to one immunoglobulin by i.v. injection of 5 mg protein, and simultaneously challenged with another in complete Freund's adjuvant, make antibody only to the class-specific determinants on the challenging antigen. Guinea pigs were given 5 mg of human γG i.v., and simultaneously challenged with mixtures of immunoglobulins included in complete Freund's adjuvant. Antisera specific for α, µ, δ and ε chains were thus produced.

This publication has 0 references indexed in Scilit: