Establishment of marek's disease lymphoblastoid cell lines from transplantable versus primary lymphomas

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Abstract
Six new Marek's disease (MD) lymphoblastoid cell lines were established in vitro by cultivation in medium containing 2‐mercaptoethanol (2‐ME). Attempts using primary lymphoma cells were generally unsuccessful; only one of 28 lymphomas yielded a cell line and that one came from an experimentally immunosuppressed chicken. In contrast, two of seven low‐passage, and two of two established MD transplantable lymphomas grew readily in vitro. A sixth line was obtained using buffy coat cells from leukemic chicken. It was concluded that the use of transplantable tumor cells and a medium containing 2‐ME provided a combination highly sulted to the establishment of cell lines from MD.