Phorbolester-treated human lymphocytes are susceptible to natural killer cell-mediated cytolysis.
Open Access
- 1 June 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 130 (6) , 2505-2507
- https://doi.org/10.4049/jimmunol.130.6.2505
Abstract
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) was found in this study to render normal human lymphocytes susceptible to allogeneic and autologous natural killer (NK) cell lysis. Both T and non-T cells became susceptible after culture for 3 days in the presence of 1 ng/ml TPA. Effector cells were nonadherent mononuclear cells of low density, enriched for large granular lymphocytes and HNK-1+ cells. Activation of effector cells with interferon increased lysis of TPA-treated lymphocytes. The present system may provide a new model to study NK cell target structures.This publication has 14 references indexed in Scilit:
- Stimulation of DNA synthesis by transient exposure of cell cultures to TPA or polypeptide mitogens: Induction of competence or incomplete removal?Journal of Cellular Physiology, 1981
- A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).The Journal of Immunology, 1981
- 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment elevates the natural killer (NK) sensitivity of certain human lymphoid linesCellular Immunology, 1981
- Natural killer cell reactivity: regulatory interactions and among phorbol ester, interferon, cholera toxin, and retinoic acid.The Journal of Immunology, 1981
- Natural killing of tumor cells by human peripheral blood cells. Suppression of killing in vitro by tumor-promoting phorbol diesters.Journal of Clinical Investigation, 1981
- Characteristics of human large granular lymphocytes and relationship to natural killer and K cellsThe Journal of Experimental Medicine, 1981
- Specific receptors for phorbol esters in lymphoid cell populations: role in enhanced production of T-cell growth factor.Proceedings of the National Academy of Sciences, 1981
- Human fetal thymus and bone marrow contain target cells for natural killer cellsEuropean Journal of Immunology, 1980
- Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester.Proceedings of the National Academy of Sciences, 1979
- Tumor promoters inhibit spontaneous differentiation of Friend erythroleukemia cells in culture.Proceedings of the National Academy of Sciences, 1977