Failure of Sugarcane Mosaic Virus to Survive in Cultured Sugarcane Tissue

Publisher Website
Google Scholar
Abstract
Explants taken from immature spindle tissues above the apical meristerm of sugarcane plants systemically infected with sugarcane mosaic virus were callused on Murashige-Skoog culture medium. Of 57 plants regenerated from this callus, 55 were virus-free as indicated by lack of symptoms after 6 mo. of growth in soil in the greenhouse and by bioassay on sorghum test plants. Bioassays of spindle tissues similar to those of the explants showed a steep infectivity gradient ranging from very low just above the growing point to very high about 4.5 cm above the growing point. The spindle tissues exhibited a reverse gradient with respect to callus formation, so that most of the callus came from tissues with the greatest infectivity Sugarcane mosaic virus was present in many, if not all, of the explants from which virus-free plants were regenerated.

This publication has 0 references indexed in Scilit: