Use of Sodium Dodecyl Sulfate in Serodiagnosis of Barley Stripe Mosaic Virus in Embryos and Leaves

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Abstract
Sodium dodecyl sulfate (SDS) was used in the serodiagnosis of barley stripe mosaic virus (BSMV) in embryos and leaves. Antisera elicited by SDS-treated preparations of partially purified BSMV were produced in rabbits and then used in an agar gel immunodiffusion medium amended with SDS. The serodiagnostic test was based on a modified Ouchterlony double-diffusion technique in which SDS replaced Leonil SA as the viral degrading agent. Filter paper disks served as seroreactant depots. The SDS-disk test worked equally well with freshly prepared or lyophilized seroreactants. More than 50,000 embryos were assayed successfully. The procedure shows promise for field surveys and seed testing worldwide.

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