cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells.
- 1 September 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (18) , 6706-6710
- https://doi.org/10.1073/pnas.85.18.6706
Abstract
A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.This publication has 29 references indexed in Scilit:
- Stimulation of macrophage tumoricidal activity by the growth and differentiation factor CSF-1Cellular Immunology, 1987
- Human CSF-1: Molecular Cloning and Expression of 4-kb cDNA Encoding the Human Urinary ProteinScience, 1987
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- Molecular Cloning of a Complementary DNA Encoding Human Macrophage-Specific Colony-Stimulating Factor (CSF-1)Science, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Role of the Conserved AAUAAA Sequence: Four AAUAAA Point Mutants Prevent Messenger RNA 3′ End FormationScience, 1984
- Survival of mononuclear phagocytes depends on a lineage-specific growth factor that the differentiated cells selectively destroyCell, 1982
- Mechanisms for the incorporation of proteins in membranes and organelles.The Journal of cell biology, 1982
- RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexaminationBiochemistry, 1977
- Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living CellsNature, 1971