A novel zinc finger transcriptional repressor, ZNF224, interacts with the negative regulatory element (AldA‐NRE) and inhibits gene expression
Open Access
- 12 December 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 534 (1-3) , 93-100
- https://doi.org/10.1016/s0014-5793(02)03783-3
Abstract
The interaction between the negative cis‐element (AldA‐NRE) and p97 repressor nuclear protein is a key step in modulating transcription of the human and mouse aldolase A (AldA) gene during the cell cycle and differentiation. In an attempt to clarify the role of transcriptional repression in regulating gene expression, we purified, from HeLa cells, the nuclear protein that specifically binds to the AldA negative regulatory element (NRE). Matrix‐assisted laser desorption ionization‐time of flight analysis and examination of protein profiles from the SwissProt database revealed that the previously defined p97 repressor is ZNF224, a zinc finger protein. We demonstrate that ZNF224, a Kruppel‐like zinc finger transcription factor, is the repressor protein that specifically binds to the negative cis‐element AldA‐NRE and affects the AldA‐NRE‐mediated transcription.Keywords
This publication has 20 references indexed in Scilit:
- Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2α subunit of NuRDGenes & Development, 2001
- Two Novel Krüppel-associated Box-containing Zinc-finger Proteins, KRAZ1 and KRAZ2, Repress Transcription through Functional Interaction with the Corepressor KAP-1 (TIF1β/KRIP-1)Journal of Biological Chemistry, 1999
- Mammalian Krüppel-like transcription factors: more than just a pretty fingerTrends in Biochemical Sciences, 1999
- Characterization of a Silencer That Modulates Transcription of the Human Distal Aldolase A PromoterBiochemical and Biophysical Research Communications, 1995
- Growth-Arrested Dependence of Aldolase A L-Type mRNA Expression in Rodent Cell LinesExperimental Cell Research, 1994
- The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression.Proceedings of the National Academy of Sciences, 1994
- Krüppel-associated boxes are potent transcriptional repression domains.Proceedings of the National Academy of Sciences, 1994
- Multiple Control Elements Regulate Transcription from the Most Distal Promoter of Human Aldolase A GeneBiochemical and Biophysical Research Communications, 1993
- In vivo activity of the most proximal promoter of the human aldolase A ene and analysis of transcriptional control elementsFEBS Letters, 1989
- Affinity purification of sequence-specific DNA binding proteins.Proceedings of the National Academy of Sciences, 1986