MICROBES AND GRAIN GERMINATION

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Abstract
The germination of grains that had been steeped in solutions/suspensions of antibiotics was improved relative to water-steeped controls. The effect of the antibiotics was most striking for dormant grain that had been steeped for 90 min rather than 60 min. By incubating decorticated grains under different amounts of water it was shown that restriction of air to decorticated dormant grains reduced germination much more than was the case with decorticated, mature grains. Incubating the dishes of grain in oxygen, rather than air, overcame the inhibitory effect of excess water, confirming that it was the restriction of the oxygen supply by the water that checked germination. It was shown that sprays of dilute sulphuric acid favoured grain germination by acting on the outer layers of the grain. When acid-sprayed and untreated samples were decorticated and incubated their germination rates under different test conditions were essentially identical. In contrast gibberellic acid was shown to exert its germination-stimulating effects and sodium hydroxide its inhibitory effects on the internal tissues. By comparing the respiration rates of ground samples of grain (entire and decorticated; untreated and surface-sterilised) it was shown that microbes in the surface layers have substantial oxygen uptake rates. It seems that the competition of these microbes with the grain tissues for oxygen is a major cause of dormancy.

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