EFFECT OF FILTRATION ON BIOLOGIC PRODUCTS .2. INFLUENCE ON CATALASE AND LIPASE ACTIVITY

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Abstract
This report emphasizes the importance of selecting a suitable filter for the sterilization of limited quantities of biologic products so that undesirable changes in content, which may be avoidable, do not result from the procedure. The effects of millipore type HA, bacterial Seitz, and ultra-fine fritted-glass filters on mycobacterial and mammalian catalase and lipase were studied. In general, using a maximal standard pressure differential of 70 cm. of mercury, small volumes of either enzyme from both sources in low concentrations were completely inactivated by Seitz filtration but were not greatly affected by the membrane filter. Results with the fritted-glass filter were variable. Corresponding, but not comparable, reductions in nitrogen content after filtration were shown.