Studies on the Growth in Culture of Plant Cells

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Abstract
Marked changes in the activities of the Embden–Meyerhof–Parnas (EMP) pathway and the pentose phosphate pathway occur during the progress of growth of sycamore cells in batch suspension culture. The activities of the two pathways were investigated by measuring the activities of six EMP pathway and four pentose phosphate pathway enzymes, and by determining the contributions of 14C from (1-14C)- and (6-14C)-glucose to CO2. During the early stages of culture both the EMP pathway and the pentose phosphate pathway make appreciable contributions to carbohydrate oxidation, but following the initiation of cell division, carbohydrate oxidation is predominantly via the EMP pathway. All the enzymes assayed were found to be associated with the soluble fraction of the cells. The regulation of carbohydrate oxidation in sycamore cells and the possible role of the pentose phosphate pathway in supplying NADPH for biosynthesis are discussed.

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