Effect of Freezing in Methylmercaptoimidazole on the Structure of Thyroglobulin

Publisher Website
Google Scholar
Abstract
The effect of methylmercaptoimidazole (and KSCN) on thyroglobulin of low and normal iodine levels in human and bovine species has been evaluated when solutions were frozen. Methylmercaptoimidazole acts like a denaturant, leading to dissociation and insolubilization of thyroglobulin. Two dissociation products are formed, the well-characterized 12S (330,000) and in human thyroglobulin a new species with a sedimentation rate of 8S (185,000). Various lines of evidence indicate that 8S is formed without cleavage of interchain disulfide bonds. In contrast to an earlier report, very little difference was found among thyroglobulins of different iodine contents in their reactivity toward methylmercaptoimidazole during freezing. (Endocrinology89: 741, 1971)

This publication has 0 references indexed in Scilit: