Evidence for N‐glycosylation and ubiquitination of the prolactin receptor expressed in a baculovirus‐insect cell system
- 22 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (2-3) , 230-234
- https://doi.org/10.1016/0014-5793(94)00772-1
Abstract
The molecular mass of the rabbit prolactin receptor (rbPRLR) deduced from cDNA cloning is 66 kDa. However, the molecular mass of the full-length receptor expressed in the insect Sf9 cells was found to be 94 kDa. In order to explain this discrepancy, we analyzed the possible post-translational modifications of the PRLR. Sf9 cells were infected with recombinant baculoviruses in the presence of tunicamycin, an inhibitor of N-glycosylation. Results showed that an additional ≈ 9 kDa of the extracellular domain could be attributed to the N-glycosylation and another additional ≈ 20 kDa covalent modification occurred in the cytoplasmic part of the receptor. Western blot analysis, using anti-ubiquitin antibodies, revealed that the rbPRLR was ubiquitinated in its cytoplasmic domain.Keywords
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