Characteristics of an Uptake System for α‐Aminoisobutyric Acid in Leishmania tropica Promastigotes1

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Abstract
L. tropica promastigotes transport .alpha.-aminoisobutyric acid (AIB), the nonmetabolizable analog of neutral amino acids, against a substantial concentration gradient. AIB is not incorporated into cellular material but accumulates within the cells in an unaltered form. Intracellular AIB exchanges with external AIB. Various energy inhibitors and sulfhydryl reagents severely inhibit uptake. The uptake system is saturable with reference to AIB and the Lineweaver-Burk plots show biphasic kinetics suggesting the involvement of 2 transport systems. AIB shares a common transport system with alanine, cysteine, glycine, methionine, serine and proline. Uptake is regulated by feedback inhibition and transinhibition.

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