Iodination-Coupled Tetrazonium and Ferric Ferricya-Nide Reduction Stains for Differentiating Red Blood Cells Containing Fetal or Adult Hemoglobin
- 1 January 1960
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 35 (4) , 205-207
- https://doi.org/10.3109/10520296009114738
Abstract
Either the iodination-coupled tetrazonium reaction or the ferric ferricyanide reduction procedure can be used to differentiate red blood cells containing fetal hemoglobin (hemoglobin F) from those containing adult hemoglobin (hemoglobin A) in blood smears. Oxalated blood is diluted with 3 parts of physiological saline, and smears are made on slides. The air-dried slides are treated with absolute ethanol for 2 min, dried, and placed in phosphate-citrate buffer of pH 3.2-3.6 for 1 min at 37°C. They are then rinsed in distilled water, and dried for storage or stained at once by either the iodination-coupled tetrazonium or the ferric ferricyanide reduction procedure. Adult hemoglobin is extracted by the buffer, so that red blood cells containing fetal hemoglobin give a much darker stain than those containing adult hemoglobin. The hemoglobin S of patients with sickle-cell anemia behaves like adult hemoglobin.Keywords
This publication has 3 references indexed in Scilit:
- FŒTAL ERYTHROCYTES IN THE MATERNAL CIRCULATIONThe Lancet, 1959
- Demonstration von fetalem H moglobin in den Erythrocyten eines BlutausstrichsJournal of Molecular Medicine, 1957
- Selective Demonstration of HistidineStain Technology, 1956