Oxygen-Linked Hydrogen Ion Binding of Human Hemoglobin. Effects of Carbon Dioxide and 2,3 - Diphosphoglycerate. II. Studies on Whole Blood

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Abstract
Acid-base titrations were performed on oxygenated and deoxygenated blood at constant PCO2. The BE-coefficient, - (δBlood-base excess)/(δHb-bound O2), is 0.3 mol/mol for normal blood, varying between 0 and 0.4 with variations in pH, PCO2, and 2,3-diphosphoglycerate concentration. The ratio between the BE-coefficient for a given plasma pH and the Haldane coefficient (-δHb-bound H+)/(δHb-bound O2)) for the corresponding erythrocyte pH is found to be 0.77. Comparison of the two coefficients indicates that (Ery-pH)=0.796 (Plasma-pH) + 1.356.