Physical and functional interaction of the archaeal single-stranded DNA-binding protein SSB with RNA polymerase
- 13 February 2004
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 32 (3) , 1065-1074
- https://doi.org/10.1093/nar/gkh259
Abstract
Archaeal transcription utilizes a complex multisubunit RNA polymerase and the basal transcription factors TBP and TF(II)B, closely resembling its eukaryal counterpart. We have uncovered a tight physical and functional interaction between RNA polymerase and the single-stranded DNA-binding protein SSB in Sulfolobus solfataricus. SSB stimulates transcription from promoters in vitro under TBP-limiting conditions and supports transcription in the absence of TBP. SSB also rescues transcription from repression by reconstituted chromatin. We demonstrate the potential for promoter melting by SSB, suggesting a plausible basis for the stimulation of transcription. This stimulation requires both the single-stranded DNA-binding domain and the acidic C-terminal tail of the SSB. The tail forms a stable interaction with RNA polymerase. These data reveal an unexpected role for single-stranded DNA-binding proteins in transcription in archaea.Keywords
This publication has 43 references indexed in Scilit:
- Basal and regulated transcription in ArchaeaBiochemical Society Transactions, 2001
- Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricusNucleic Acids Research, 2001
- The archaeal TFIIEα homologue facilitates transcription initiation by enhancing TATA‐box recognitionEMBO Reports, 2001
- [19] Preparation of components of archaeal transcription preinitiation complexPublished by Elsevier ,2001
- The orientation of DNA in an archaeal transcription initiation complex.Nature Structural & Molecular Biology, 2000
- The Role of Transcription Factor B in Transcription Initiation and Promoter Clearance in the Archaeon Sulfolobus acidocaldariusJournal of Biological Chemistry, 2000
- Structure and functional relationships of archaeal and eukaryal histones and nucleosomesArchiv für Mikrobiologie, 2000
- Interaction of E. coli Single-Stranded DNA Binding Protein (SSB) with Exonuclease I. The Carboxy-Terminus of SSB Is the Recognition Site for the NucleaseBiological Chemistry, 2000
- Adaptations of the archaeal cell membrane to heat stressFrontiers in Bioscience-Landmark, 2000
- Orientation of the transcription preinitiation complex in ArchaeaProceedings of the National Academy of Sciences, 1999