Nucleotide sequence of the N-acetylneuraminate lyase gene of Escherichia coli.

Abstract
We have established the sequence of a DNA fragment containing the Escherichia coli N-acetylneuraminate lyase gene (nanA or npl). The coding sequence has an open reading frame of 297 amino acids. Amino acid compositions obtained by analysis of amino acids were mostly consistent with those calculated from the DNA sequence. The open reading frame contains a codon sequence of 15 amino acids deduced to be identical to the known N-terminal amino acid sequence of N-acetylneuraminate lyase. The first methionine was removed from the enzyme because the N-terminal amino acid was alanine.

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