A Simple, Rapid, High-resolution Chromosome Technic for Lymphocytes

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Abstract
Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 m KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.

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