Lanthanide Luminescence Detection of Bleomycins and Nalidixic Acid

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Abstract
Bleomycins and nalidixic acid transfer energy to Tb(III). Subsequent emission from Tb(III) provides for a selective and sensitive detection method. Temperature studies indicate that energy transfer for both compounds involves an intramolecular process. The efficiency of reversed-phase separations of bleomycins is improved by adding Tb(III) to the mobile phase. Tb(III) luminescence detection is compatible with a wide range of mobile phases suitable for the separation of nalidixic acid. Detection limits for bleomycin A2 and nalidixic acid are 3 × 10−6 M and 4 × 10−7 M respectively.