GASEOUS LOSSES OF NITROGEN FROM GREEN PLANTS. II. STUDIES WITH EXCISED LEAVES IN NUTRIENT MEDIA

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Abstract
Sterilized leaves of Iris, Belamcanda and Narcissus were floated on a sugar-mineral medium and exposed to artificial light for 60-84 hrs. to determine if gaseous losses of N occur. The excised leaves actively assimilated the N of ammonium nitrate, potassium nitrate and ammonium chloride but no significant loss of N occurred. The results, therefore, fail to confirm the observations of Pearsall and Billimoria, who reported that Narcissus leaves brought about large losses of N where kept under similar conditions. There is little to indicate that appreciable loss of gaseous N occurs in plant tissues as a result of the reaction of nitrous acid with amines or ammonia.
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